FAK ELISA Kit

Produktnummer ABIN2043495

Dieses Human FAK ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human FAK zu quantifizieren.

Kurzübersicht für FAK ELISA Kit (ABIN2043495)

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails FAK ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Marke: BioAssay™

Sensitivität: < 0.53 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Focal Adhesion Kinase (FAK) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Focal Adhesion Kinase (FAK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Focal Adhesion Kinase (FAK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Focal Adhesion Kinase (FAK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Focal Adhesion Kinase (FAK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Andere Bezeichnung: Focal Adhesion Kinase (FAK)

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling